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耐高温高产酒精酵母的筛选及其大曲生产应用研究(107)
作者： 王 珍，张永利，孟勤燕，陈 雪，齐 欢，李浩浩，闫宗科
| 地址：陕西西凤酒股份有限公司，陕西 凤翔 |
邮编：721406 |
| 摘要： |
以凤型大曲为菌种来源，采用三级筛方法分离筛选耐高温高产酒精酵母，并对混合菌种模拟固态发酵结果进行比较分析，将最优组合应用于大曲实际生产。结果表明：（1）三级筛选后共优选出6株目的菌株，其中耐44 ℃的酵母菌2株，为Y3和Y22，耐42 ℃的酵母菌4株，为Y7、Y10、Y13及Y14；（2）不同菌株混合固态发酵均能有效提高酒醅酒精度含量，其中6株菌混合发酵时，酒醅酒精度可达5.85 %vol，较对照提高31.4 %；（3）6株菌应用于大曲生产，出房曲糖化力、液化力、发酵力、酯化力分别提升了14.9 %、13.1 %、14.2 %和5.1 %。总体来看，试验筛选获得的6株菌混合使用能够有效提升大曲的综合性能，具有较好的应用前景。 |
| 关键词： |
耐高温； 产酒精； 酵母； 大曲； 白酒 |
| 中图分类号：
TS261.2；TS261.7；TS262.3 |
文献标识码：
A |
文章编号：
1001-9286（2020）01-0107-08 |
英文标题：
Screening of Thermotolerant Yeast Strains with High-Yield of Alcohol and Its Application in Daqu Production
英文作者：
WANG Zhen, ZHANG Yongli, MENG Qinyan, CHEN Xue, QI Huan, LI Haohao and YAN Zongke
英文地址：
Xifeng Distillery Co. Ltd., Fengxiang, Shaanxi 721406, China
| 英文摘要： |
Fengxiang Daqu was used as the strain source and three-step screening process was adopted for the screening of thermotolerant strains with high-yield of alcohol. Then the simulated solid-state fermentation results of mixed strains were compared and the best strain combination was used in Daqu production. The results showed that, (1) six target strains were obtained, of which 2 strains (Y3 and Y22) were resistant to 44 °C, 4 strains (Y7,Y10,Y13 and Y14) were resistant to 42 °C; (2) all mixed combinations of strains could effectively improve the ethanol content of fermented grains; as the 6 strains were mixed, the alcohol content could reach up to 5.85 %vol, increasing by 31.4 % compared with the control; (3) the use of the 6 strains in Daqu production could improve the saccharifying power, liquefying power, fermenting power and esterifying power of Daqu by 14.9 %, 13.1 %, 14.2 % and 5.1 % respectively. In general, the mixed use of the 6 strains could practically improve the comprehensive performance of Daqu, which had a good application prospect. |
| 英文关键词： |
thermotolerant; alcohol-producing; yeast; Daqu; Baijiu |
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