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刊物期号: 201703

青稞酒中糖精钠含量的测定方法研究(107)

作者: 蔡林森

地址:青海省产品质量监督检验所,青海 西宁 邮编:810001
摘要 建立了高效液相色谱法测定青稞酒中糖精钠的检测方法。将青稞酒样品10.0 g在平行蒸发仪上减压蒸发至近干再用超纯水定容至10 mL,待分析。采用Inertsustain C18(4.6×250 mm×5 μm)柱分离。以0.02 mol/L的乙酸铵缓冲液和甲醇(V∶V=85∶15)为流动相,流速0.8 mL/min,柱温40 ℃,进样量为10 μL,紫外检测器的波长为220 nm。实验结果表明,糖精钠在5~150 μg/mL范围内线性良好,线性方程为:y=24893.5x,相关系数R2=0.9999。加标回收率为97.74 %~106.9 %,检出限为0.023 μg/mL。本方法简便、快速、精密、准确,可用于青稞酒中糖精钠的检测。
关键词 糖精钠; 青稞酒; 高效液相色谱
中图分类号: TS262.3;TS261.7 文献标识码: A 文章编号: 1001-9286(2017)03-0107-02

英文标题: Determination of Saccharin Sodium Content in Highland Barley Liquor

英文作者: CAI Linsen

英文地址: Qinghai Product Quality Supervision and Inspection Institute, Xining, Qinghai 810001, China

英文摘要 The determination method of saccharin sodium content in highland barley liquor by HPLC had been developed. 10.0 g liquor sample was placed on multivapor for decompression-evaporation until almost dry, then ultra-pure water was injected to 10 mL for subsequent analysis. Inertsustain C18 (4.6 × 250 mm × 5 μm) column was used for separation. 0.02 mol/L ammonium acetate buffer and methanol (V/V = 85∶15) was used as mobile phase, the flowing rate was 0.8 mL/min, the column temperature was at 40 ℃, the injection volume was 10 μL, and the wavelength of ultraviolet detector was at 220 nm.The experimental results showed that sodium saccharin showed a good linear relationship within 5 to 150 μg/mL and the linear equation was y=24893.5x, the correlation coefficient R2=0.9999, the recovery was between 97.74 % to 106.9 %, and the detection limit was 0.023 μg/mL. This method was simple, rapid, precise, accurate and it could be used in the analysis of saccharin sodium in highland barley liquor.
英文关键词 saccharin sodium; highland barley liquor; liquid chromatography
      

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