建立了高效液相色谱法测定青稞酒中糖精钠的检测方法。将青稞酒样品10.0 g在平行蒸发仪上减压蒸发至近干再用超纯水定容至10 mL，待分析。采用Inertsustain C18（4.6×250 mm×5 μm）柱分离。以0.02 mol/L的乙酸铵缓冲液和甲醇（V∶V=85∶15）为流动相，流速0.8 mL/min，柱温40 ℃，进样量为10 μL，紫外检测器的波长为220 nm。实验结果表明，糖精钠在5～150 μg/mL范围内线性良好，线性方程为：y=24893.5x，相关系数R2=0.9999。加标回收率为97.74 %～106.9 %，检出限为0.023 μg/mL。本方法简便、快速、精密、准确，可用于青稞酒中糖精钠的检测。 |
糖精钠； 青稞酒； 高效液相色谱 |
Determination of Saccharin Sodium Content in Highland Barley Liquor
Qinghai Product Quality Supervision and Inspection Institute, Xining, Qinghai 810001, China
The determination method of saccharin sodium content in highland barley liquor by HPLC had been developed. 10.0 g liquor sample was placed on multivapor for decompression-evaporation until almost dry, then ultra-pure water was injected to 10 mL for subsequent analysis. Inertsustain C18 (4.6 × 250 mm × 5 μm) column was used for separation. 0.02 mol/L ammonium acetate buffer and methanol (V/V = 85∶15) was used as mobile phase, the flowing rate was 0.8 mL/min, the column temperature was at 40 ℃, the injection volume was 10 μL, and the wavelength of ultraviolet detector was at 220 nm.The experimental results showed that sodium saccharin showed a good linear relationship within 5 to 150 μg/mL and the linear equation was y=24893.5x, the correlation coefficient R2=0.9999, the recovery was between 97.74 % to 106.9 %, and the detection limit was 0.023 μg/mL. This method was simple, rapid, precise, accurate and it could be used in the analysis of saccharin sodium in highland barley liquor.
saccharin sodium; highland barley liquor; liquid chromatography