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刊物期号: 201604

核苷功能菌腺苷超声提取工艺优化(70)

作者: 周新虎,陈 翔,严启梅,杨 勇,李 喆,姜 勇,戴 源,谭洪娣,宋 宝

地址:江苏洋河酒厂股份有限公司,江苏 宿迁 邮编:223800
摘要 研究超声波水提法提取核苷功能菌腺苷的最佳工艺。通过单因素试验和正交试验对提取工艺进行优化设计,采用高效液相色谱法测定腺苷含量,考察料液比、超声提取温度、超声提取时间和超声功率对核苷功能菌腺苷含量的影响。 得出影响核苷功能菌腺苷提取效果的先后次序为:提取时间>料液比>超声功率>提取温度。最佳提取工艺条件为料液比1/150 g/mL、提取温度55 ℃、提取时间20 min、超声功率350 W。该工艺条件下,核苷功能菌菌丝体中腺苷含量高达435.58 mg/100 g。
关键词 微生物; 核苷功能菌; 腺苷; 超声提取法; 正交试验设计
中图分类号: TS262.3;Q93-3;TS261.1;TS261.7 文献标识码: A 文章编号: 1001-9286(2016)04-0070-04

英文标题: Optimization of Ultrasonic-assisted Extraction of Adenosine from Nucleoside Functional Bacteria

英文作者: ZHOU Xinhu, CHEN Xiang, YAN Qimei, YANG Yong, LI Zhe, JIANG Yong, DAI Yuan, TAN Hongdi and SONG Bao

英文地址: Yanghe Distillery Co.Ltd., Suqian, Jiangsu 223800, China

英文摘要 The ultrasonic-assisted extraction conditions of adenosine from nucleoside functional bacteria were optimized by single factor test and orthogonal experiments. Adenosine content was measured by HPLC. The effects of solid-liquid ratio, extraction temperature, ultrasonic extraction time and ultrasonic power on adenosine content were investigated. The factors influencing adenosine extraction ranked in decreasing sequence as follows: extraction time>solid-liquid ratio>ultrasonic power>extraction temperature. The optimum technical parameters were summed up as follows: solid-liquid ratio was 1/150 g/mL, extraction temperature at 55 ℃, ultrasonic extraction time was 20 min, and ultrasonic power was 350 W. Under above conditions, the content of the extracted adenosine from nucleoside functional bacteria reached up to 435.58 mg/100 g.
英文关键词 microbe; nucleoside functional bacteria; adenosine; ultrasonic-assisted extraction; orthogonal experiments design
      

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