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刊物期号: 201604

响应面法优化酵母增殖培养基(47)

作者: 牛希跃1,2,任晓镤1,2,叶 林1,2,兰鸿珠1,许 倩1,2

地址:1.塔里木大学生命科学学院,新疆 阿拉尔 843300; 2.南疆特色农产品深加工兵团重点实验室,新疆 阿拉尔 843300
摘要 为评价培养基成分(碳源、氮源、生长因子等)对酵母菌生长量的影响,利用Plackett-Burman 实验研究主要影响因素,经最陡爬坡实验确定各因素峰值,再利用响应面法优化主要影响因素。结果表明,最适培养基配方为蔗糖58 g/L、苹果汁22 mL/L、蛋白胨12 g/L、马铃薯汁10 mL/L、酵母粉10 g/L、氯化钙0.1 g/L、磷酸氢二钾1 g/L,在pH4.5条件下28 ℃培养24 h,酵母菌的生长量为8.8748 cfu/mL。优化后的培养基与传统的YPD培养基(8.5543 cfu/mL)相比,酵母菌菌数提高明显。
关键词 酵母; 培养基; PB设计; 响应面
中图分类号: TS261.1;TS262.3;Q93-3 文献标识码: A 文章编号: 1001-9286(2016)04-0047-04

英文标题: Optimization of Yeast Culture Medium by Response Surface Method

英文作者: NIU Xiyue1,2, REN Xiaopu1,2, YE Lin1,2, LAN Hongzhu1 and XU Qian1,2

英文地址: 1.College of Life Sciences, Tarim University, Alar, Xinjiang 843300; 2. Key Lab of Deep-processing of Special Agricultural Products, Alar, Xinjiang 843300, China

英文摘要 In order to evaluate the effects of culture medium compositions (carbon source, nitrogen source, growth factors etc.) on the growth rate of yeast, the main influencing factors were determined by Plackett-Burman design. On the basis of the most steep climb path experiment, the peak values of those factors were determined and those factors were then optimized by response surface method as follows: sucrose 58 g/L, apple juice 22 mL/L, peptone 12 g/L, potato juice 10 mL/L, yeast powder 10 g/L, calcium chloride 0.1 g/L, dipotassium hydrogen phosphate 1 g/L, and the growth rate of yeast was 8.8748 cfu/mL after 24 h culture at 28 ℃ and pH 4.5. Compared with traditional YPD culture medium (8.5543 cfu/mL), yeast quantity in the optimized culture medium increased by 2 times.
英文关键词 yeast; culture medium; PB design; response surface method
      

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